![]() ![]() Recommended volume per well for 1.0 mm mini gelġ0 μL for electrophoresis, 5 μL for blottingĥ μL for electrophoresis, 2. ~160, 120, 80, 60, 50, 40, 30, 20, 15, 10 kDaĮach protein standard contains a 6X His-tag enabling the detection with the InVision His-tag In-gel StainĬolorimetric or methods that detect phosphorylated proteins such as Pro-Q Diamond phosphoprotein gel stainsĬolorimetric or methods that detect glycosylated proteins such as Pro-Q Glycoprotein stain kits Chemiluminescent Western blots are popular assays for assessing protein expression. As originally conceived by Towbin et al. ~1236, 1048, 720, 480, 242, 146, 66, 20 kDa Western blotting is a powerful and commonly used tool to identify and quantify a specific protein in a complex mixture. In: 2D PAGE: Sample Preparation and Fractionation. A high-affinity reversible protein stain for Western blots. Peppermint Stick Phosphoprotein Molecular Weight StandardsĬand圜ane Glycoprotein Molecular Weight Standards A chemiluminescent blot is optimally imaged after 35 minutes of incubation with the substrate and may produce a signal for several hours. Tagged- proteins in the 10 -250 kDa range contain a Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II sequenceĭirect visualization can be achieved through standard staining protocols (e.g., Coomassie, etc.) Tagged-proteins contain an integral Strep-tag™ II sequence and can be detected on western blots using Strep-Tactin™ conjugates or an antibody against Strep-tag™ II sequence Just load your samples and reagents into the microplate, and Jess does the rest. PageRuler Unstained Broad Range Protein LadderĪccurate estimation across a broader range Jess automates the protein separation and immunodetection of traditional Western blotting, eliminating many of the tedious, error-prone steps. Recommended Volume per well for 1.0 mm gel (μL)Ĭolorimetric, NIR fluorescence (700nm channel, blue bands), RGB fluorescence (550 nm channel, orange bands) Consisting of recombinant proteins fused to an IgG binding site, these versatile molecular weight ladders bind the primary or secondary antibody used for the detection of the target protein. Visible monitoring of gel separation and transfer efficiency Western blot protein ladders are designed for protein molecular weight estimation directly on the blot during detection. Spectra Multicolor Broad Range Protein Ladderīest multicolor prestained ladder for routine applicationsĤ colors for improved visualization during separation and transferĪnalysis of high molecular weight proteins
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